--- tags: bioinformatics --- # Running nfcore pipelines on Tufts HPC Shirley Li, Bioinformatician, TTS Research Technology xue.li37@tufts.edu Date: 2024-11-01 You have two options for running the nf-core pipeline on Tufts HPC: through Open OnDemand or the command-line interface. ## 1. Open Ondemand Navigate to [Open Ondemand](https://ondemand.pax.tufts.edu/), under `Bioinformatics Apps`, choose the appropriate application for the pipeline you wish to execute. ## 2. Linux Command Line Interface By using `module avail`, you can see the available modules. There are two ways to run the nf-core pipeline via the command-line interface: ### Option 1 Load the necessary modules ``` module load singularity/3.8.4 module load nf-core/2.13.1 ``` Run the pipeline with this command: ``` nextflow run nf-core/mag -profile tufts ... ``` For a comprehensive list of available pipelines, visit the [nf-core website](https://nf-co.re/pipelines). There are currently 107 pipelines available through nf-core. ### Option 2 Load singularity module and the specific pipeline module: ``` module load singularity/3.8.4 module load nf-core-mag/2.5.4 ``` Execute the pipeline using: ``` mag ... ``` Currently, 31 pipelines have been deployed as modules on Tufts HPC for ease of use.