.. _backbone-label: ######## Medaka ######## ************** Introduction ************** Medaka is a tool to create consensus sequences and variant calls from nanopore sequencing data. ********** Versions ********** - 1.11.1 ********** Commands ********** - medaka - medaka_consensus - medaka_counts - medaka_data_path - medaka_haploid_variant - medaka_version_report ************* Example job ************* Adjust slurm options based on job requirements (`slurm cheat sheet `_): .. code:: #!/bin/bash #SBATCH -p partitionName # batch, gpu, preempt, mpi or your group's own partition #SBATCH -t 1:00:00 # Runtime limit (D-HH:MM:SS) #SBATCH -N 1 # Number of nodes #SBATCH -n 1 # Number of tasks per node #SBATCH -c 4 # Number of CPU cores per task #SBATCH --mem=8G # Memory required per node #SBATCH --job-name=medaka # Job name #SBATCH --mail-type=FAIL,BEGIN,END # Send an email when job fails, begins, and finishes #SBATCH --mail-user=your.email@tufts.edu # Email address for notifications #SBATCH --error=%x-%J-%u.err # Standard error file: --.err #SBATCH --output=%x-%J-%u.out # Standard output file: --.out module purge ### Optional, but highly recommended. module load medaka/XXXX ### Latest version is recommended.